Electron microscopy
Microtome for Specimen-Sectioning
- Practical Electron Microscopy and Database -
- An Online Book -
Microanalysis | EM Book                                                                                   https://www.globalsino.com/EM/        

This book (Practical Electron Microscopy and Database) is a reference for TEM and SEM students, operators, engineers, technicians, managers, and researchers.


A microtome for specimen-sectioning is a tool used to cut extremely thin slices from bulk material. Specimens in thickness between 50 nm and 100 µm can be prepared for both transmission light microscopy and transmission electron microscopy. This method use steel, glass, or diamond blades depending on the specimen being sliced and the desired thickness of the sections needed. For instance, steel blades are often used to prepare sections of animal or plant tissues for light microscopy histology, glass knives are used to slice sections for both light microscopy and electron microscopy. In industry, grade diamond knives are used to slice hard materials such as bone, teeth and plant matter for both light microscopy and electron microscopy. Note that the materials might damage the knives.

For particles and powders, sectioning can be performed by embedding the particles in resin. In this case, the hardness of the resin should be adjusted to match that of resin in order to avoid the particles, e.g. pine seeds, coming out from the resin during sectioning.



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