Practical Electron Microscopy and Database

The low spherical aberration of the objective lens gives a smaller point resolution as well as produces fewer oscillations of the contrast transfer function (CTF) beyond the point resolution. The accurate determination of the spherical aberration coefficient C_s is very important to many applications of EM techniques, such as electron holography [1,2], image processing and deconvolution [3], and electron crystallography of proteins [4].

1. Liehte, H., Parameters for high-resolution electron holography, Ultramicroscopy, 1993, 51, 15.
2. Peng, L.-M., Ren, G., Duan, X. F., Samplling theorem and digital electron microscopy, J. Chinese Electron Microscopy Society (in Chinese), 1996, 15(2-4), 117.
3. Li, F. H., Combination of high resolution electron microscopy and electron diffraction in crystal structure determination, J. Chin. Electr. Microsc. Soc., 1996, 1592-4, 143.
4. Baumeister, W., Typke, D., Electron Crystallography of Proteins, State of the Art and Strategies for the Future, MSA Bulletin, 1993, 23(1), 11.