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Examples of parameters of .locateCenterOnScreen: region = (320, 860, 1320, 1075), grayscale = True, confidence = 0.8.

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Pyautogui uses screencaps to find the location of stuff on the screen. he problem on macs with a retina display is that the screen output have double the number of pixels, to take advantage of the system’s greater pixel density for a crisper image.Therefore, when you take a screenshot, you get an image containing double the number of pixels than the same image from a non-retina Mac

locateCenterOnScreen can give wrong coordinatess because of the reasons below:
         i) MacBook Pro does not provide correct coordinatess, e.g. the actual screen resolution on MacBook is 2880 x 1800, but pyautogui size() returns 1920 x 1080.
         ii) "Transparency Effects" in "Themes and Related Settings => Colors" has been turned on Windows 10.
         iii) Some format of PNG, JPG and GIF does not work on some PC due to 'pixel-perfect' requirement.
         iv) pyautogui image recognition does not working properly on some images.

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Click the first match continuously: code:
         

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Click the link on a webpage with a similar feature: code:
         
Template:         
         
Webpage for click:         
         
Output: opened webpage (only clicked the closest one in red):         
         
Printed output (only printed the closest one in red above):     
             

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Exception for locateCenterOnScreen: code:
         
Output:
         

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Load images and ColorMixing in DigitalMicrograph: code:          
         
Output:         
         
         
         

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Locate center on screen by locateCenterOnScreen() with reference options: code:
         
4651d.png:         
         
4651e.png:         
         
4651g.png:         
         
Output (image1 and image2 are blocked by the IDLE window, then it looked for image3):         
         

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Reverse function of locateCenterOnScreen(): code:
         
Original image on screen :         
         
Matched and opened image: