Electron microscopy
Troubleshooting Related to GIF Software and Hardware
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If the FilterControl software in the GIF (Gatan Imaging Filter) system can not recognize the hardware, the following steps need to be verified in trouble shooting process:
         i) The Power Supply and Gatan Instrumentation Bin are turned on.
         ii) The Gatan Instrumentation Bin (GIB) and the Gatan FilterControl computer are correctly connected each other.
         iii) If no GIF image (or spectrum) is obtained on the computer screen (or live TV), the relevant trouble shooting steps should be performed.

If the GIF stops working some time after it is turned on, then we need to do the troubleshooting as follows:
         i) The GIF lenses may be or had been overheating so that the protection circuitry shut the lenses off. In this case, we need to check the water cooling. The flow indicator should be set to 0.05 gallons (0.19 liters) per minute. Furthermore, to verify that the water-flow indicator is not stuck at a reading, we can adjust the regulator valve to see if the flow rate changes or not.
         ii) The fan in the GIB is running.
         iii) Once we made sure the cooling is back to working correctly, the DigitalMicrograph interface (or plus the PC) needs to be restarted in order to turn the lenses back on.
         iv) Measure the power supply voltages at the test points either on the back or inside the GIB. They should be within 10% of the values listed in the GIF user manual.

EELS only captures zero loss peak (ZLP) in the DM software. When a higher energy range is shifted, the spectrum simply shifts the ZLP to the that energy. In this case, the solution can be
         i) Adjust the drift tube.

If the energy-selecting slit is bad or contaminated, then:
         i) Spectrum profile and mapping are not affected since the slit is withdrawn in spectroscopy mode.
         ii) Lines and (or) bands can exist on EFTEM images when the slit is inserted.
         iii) Lines and (or) bands are weak at low loss energies, especially at zero-loss, since the image intensity is strong, but stronger at high loss energies.
         iv) The lines and (or) bands are much clear on EFTEM images when a smaller energy window is used.
         v) The lines and bands cannot be removed by performing “Remove dark references”.