Chapter/Index: Introduction | A |
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Appendix
Software Programs for TEM Holography
| For TEM holography data acquisition and analysis, several specialized software programs are commonly used to handle the unique requirements of electron holography, including phase reconstruction and image processing:
- Gatan DigitalMicrograph: Widely used for TEM and STEM image acquisition and analysis, this software is highly compatible with Gatan cameras and provides tools for holography. DigitalMicrograph allows for the analysis of phase and amplitude images and supports plugins for advanced processing.
- HoloWorks: Developed specifically for electron holography, HoloWorks provides tools for reconstructing holograms, calculating phase and amplitude images, and performing quantitative analysis on electron holograms. It is commonly used in academic and research settings where precise holography data analysis is required.
- Velox: This software by Thermo Fisher Scientific (formerly FEI) supports electron microscopy data acquisition and provides phase reconstruction capabilities for holography data. Velox is compatible with Thermo Fisher's TEM systems and integrates various imaging modes.
- Python-based custom scripts: Researchers often use Python libraries, such as NumPy, SciPy, and OpenCV, to create custom workflows for processing holography data. Python is particularly useful for advanced, tailored analysis or when combining holography with other image-processing techniques.
- MATLAB: Many researchers use MATLAB for holography data processing, especially for custom phase reconstruction and quantitative analysis. MATLAB’s image processing and signal analysis toolboxes provide a flexible environment for processing holographic images.
These tools allow for the detailed analysis required in TEM holography, including phase unwrapping, fringe analysis, and correction of distortions to accurately represent the specimen's electrostatic or magnetic fields.
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