Electron microscopy
Calibration of Magnification and Scale Bar in TEM
- Practical Electron Microscopy and Database -
- An Online Book -
Microanalysis | EM Book                                                                                   http://www.globalsino.com/EM/        

This book (Practical Electron Microscopy and Database) is a reference for TEM and SEM students, operators, engineers, technicians, managers, and researchers.


In TEM measurements, the magnification of the image-forming lens system has normally an error of 5% - 10% so that the final magnification has an error of 5%-10%. To obtain more accurate magnification (and thus dimension measurement), e.g. in HRTEM, the magnification should be calibrated with a lattice image of a known standard specimen by recording both the specimen and standard (e.g. evaporated gold particles) in the same field of view. Furthermore, the instruments may give a few percent of image distortion which also needs to be calibrated.



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