Low Voltage EMs in Biological Applications
- Practical Electron Microscopy and Database -
- An Online Book -

http://www.globalsino.com/EM/



 

 

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Strong scattering for low energy electrons leads to higher contrast, so voltages of 60 to 80 kV are preferred for low-contrast biological specimens.

However, in some cases (e.g. some organic systems in biology field), lower voltage TEM and STEM cannot significantly reduce beam damage since knock-on damage is not the main mechanism for such materials. In these cases, the electron dose is the most important factor on specimen damage, and thus lower dose is the best choice.

 

 

 

 

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